Micropropagation insures a good regular supply of medicinal plants, using minimum space and time 37. Protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants. The regeneration of shoot tips from the fieldgrown plants and in vitro shoots placed horizontally differed. Primarily it is widely used in a wide sense to in vitro culture of plant cells, tissues as well as organs. In the present study, we have developed an in vitro propagation protocol for g. In vitro propagation and comparative phytochemical analysis. In vitro regeneration of medicinal plants hold tremendous potential for the production of highquality plant based medicine tripathi and tripathi, 2003. This practice when performed nonsustainably threatens species and populations. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation.
In vitro and in vivo studies on selected medicinal plants 100 5. In vitro propagation of various threatened medicinal plants with anticancer activity. An overview 25 transferred to the field conditions52. Environment can be controlled or altered to meet specific needs of the plant. Observations were recorded on the percent survival of rooted and acclimatized plants. It is a medicinal plant native to asia widely used in ayurveda. In vitro flowering and rapid propagation of vitex negundo. Plant in vitro regeneration is a biotechnological tool that offers a potential solution to the problem of medicinal plants decimation in south africa. It is a cost effective technique and could save labor, time and energy in plant propagation system. Micropropagation of tashnedari endangered medicinal plant. Conservation of genetic material of many threatened medicinal plants also involves culturing techniques.
In vitro propagation in many cases is more rapid than traditional. Pdf in vitro micro propagation of medicinal plant abroma. Tissue culturing of medicinal plants is widely used to produce active compounds for herbal and. In vitro propagation offers a good alternative to multiply novel and endangered plant species. Thus, the paper reports the successful in vitro propagation of commiphora wightii. A biotechnological tool capable of solving the problem of medicinal plants decimation in south africa aj afolayan department of botany, university of fort hare, private bag x14, alice 5700, south africa. In vitro manipulation and propagation of medicinal plants. Mediumterm storage for some medicinal plant species collected from taif governorate was developed. The present investigation demonstrated the in vitro regeneration and multiplication potential of this plant and the various factors affecting the in vitro propagation are reported.
This study reports an improved protocol for in vitro shoot multiplication and ex vitro acclimation of bupleurum kaoi, an endangered medicinal herb. In vitro mass propagation from shoot tip explants of. Protocols for establishment of an in vitro collection of medicinal plants in the genus scutellaria 155 ian. In vitro plantlet production system for kaempferia galanga. Chuakul, an endangered species of stemonaceae, was established. In vitro plants are exposed to invariably controlled growth conditions such as high amount of organic and inorganic nutrients, plant growth regulators, carbon source, high humidity, low light and poor gaseous exchange. Tissue culturing of medicinal plants is widely used to produce active compounds for herbal and pharmaceutical industries. In vitro propagation of two tuberous medicinal plants.
In vitro propagation of the medicinal plant ziziphora. Samaras g, tantsi a, papanastasi k, grigoriadou k, maloupa e 2012 in vitro propagation of the native aromatic medicinal plants sideritis scardica griseb. This work aims to develop a reliable protocol for the in vitro propagation of z. Repeated subculturing of the shoot tips and single nodes at 6week intervals for over a year in combination of 4. The full retraction notice in pdf is preceding the original paper which is marked retracted. The explants are cultured on ms medium containing different concentration and combinations of growth regulators like 6benzylaminopurine. The advantages of in vitro micro propagation of medicinal plant are listed below. Antibacterial activity in vitro of medicinal plants.
As a vital chemical component, pgrs regulate various physiological and developmental processes during the. The apical meristems was used as the explants cultured on murashige and skoog 1962 medium ms supplemented different concentrations and. Pdf in vitro micropropagation of medicinal plants by tissue culture. Factors affecting in vitro propagation of momordica balsamina. As per iucn red list version 20162, from 1996 to 2016 numerous medicinal plants with prospective uses are facing risk of extinction due to lack of conservation knowledge, extensive exploitation by pharmaceutical industries, habitat loss and deforestation. Proc of the 25th conference of the hellenic society for horticultural science, 14 november 2011, limassol, cyprus, vol 2. Chapter ii in vitro propagation techniques and medicinal. Micropropagation of medicinal plants has been achieved through rapid proliferation of shoottips and axillary buds in culture. In vitro propagation and conservation of zeyheriamontana mart. In vitro cloned important medicinal plants grown ex vitro plant species comman name explants ex vitro cultivation reference allium sativum l.
The present study aimed to establish an efficient protocol for micropropagation of l. An illustrated, scientific and medicinal approach appears to be a medical compendium of plants intended as a guide and reference resource for professionals in the field. Propagation, medicinal plants introduction the term tissue culture also referred to as cell grown in vitro or sterile culture media, has great significance in both basic and applied studies. In vitro propagation of the medicinal plant ziziphora tenuior. A protocol for rapid in vitro propagation using nodal explants obtained from 2yrold, fieldgrown medicinal plants of plumbago zeylanica l. Salim zaid, a mohamad suleiman, a sami abbas, b and michael wink c. Propagation, medicinal plants introduction the term tissue culture also referred to as cell grown in vitro or sterile culture. Species propagated include those that are listed as critically endangered and as vulnerable in the red list of threatend plant species. Supplementing 2ip to media, the survival rate increased from 70% to 100%. Plant tissue culture offers tremendous opportunities in plant propagation, plant improvement and production of plants with desirable agronomical features. Antibacterial activity in vitro of medicinal plants wardah shahid 1, rabia durrani 2, saira iram2, maryam durrani 3, fawad ali khan 4 1plant taxonomy lab, department of plant sciences, faculty of biological sciences, quaidiazam university, 45320, 00925190643194, islamabad, pakistan.
In vitro propagation and conservation of zeyheria montana mart. Moreover, it is also used to provide sufficient number of plantlets for planting from few stock plants, which does not produce seeds or does not respond well to vegetative propagation 10. Factors affecting in vitro propagation of momordica. Worldwide, many medicinal and aromatic plants maps are still collected from the wild and only a small fraction of them are exclusively sourced from cultivation. It is a medicinal plant native to asia widely used in ayurveda, homeopathy, unani and siddha medicines. In vitro propagation techniques and medicinal plant species. Here, we established the protocol for plant regeneration of psoralea corylifolia via invitro micro propagation. An overview 29 sharon and d souza reported that the size of the nodal explant has an important role in producing multiple shoots from the nodal explants of bixa orellana and nodal explants having a length of 0. Tissue culturing of medicinal plants is widely used to produce active compounds for. It is a medicinal plant native to asia widely used in ayurveda, homeopathy, unani and siddha. The gradual decline in the population of psoralea corylifolia demand. Ex vitro rooting could enhance the chances of survival of plantlets in the field conditions.
A rapid clonal propagation system for kaempferia galanga zingiberaceae, a rare folk medicinal herb has been developed. An overview of tissue culture studies on important chinese medicinal plants and related species is presented. In vitro mass propagation from shoot tip explants of vernonia cinerea l. Plants are widely used by all sections of the society either as folk medicines or as pharmaceutical preparation of modern medicine. Ex situ preservation for some endemic and rare medicinal. Withania somnifera is an important medicinal plant and used worldwide in pharmaceutical industry.
Micropropagation, nodal explant, ms medium, kinetin, benzylaminopurine, c. Medicinal plants are of great interest in biotechnology. Micro propagation of tashnedari endangered medicinal plant in vitro culture. The indigenous species selected for in vitro propagation include those species that display poor rooting of cuttings, and known to produce low seed numbers and have relatively short seeding seasons. Nodal and internodal explants were cultured on murashige and skoog ms medium supplemented with thidiazuron tdz alone or in combination with 1naphthaleneacetic acid naa. It is found in arid, rocky tracts of rajasthan and. Although withania propagates vegetatively in its natural state, but propagation.
Many endangered plants are tissue cultured by callus growth and somatic embryogenesis. In vitro and in vivo studies on selected medicinal plants. In vitro propagation of medicinal and aromatic plants. Lamiaceae is an aromatic herb used for its medicinal values against fungi, bacteria. In vitro propagation of the endangered medicinal tree arbutus. In the present study, a procedure for in vitro propagation of this endangered herb through somatic embryogenesis has been established. The application of encapsulated shoot tips and nodal segments may contribute to the. In vitro propagation of plants can be influenced by a variety of factors that are categorized either as environmental or hormonal plant growth regulators pgrs. In vitro regeneration of shoots and ex vitro rooting of an. There has been an ever expanding market of herbs and herbal based medicinal preparations all over the world. In vitro plant propagation laboratory technical capacity 22 laminar flow work benches 4 independent dissection rooms 5 growth rooms each capacity 1m plantannum separate quarantine area and growth room for imported plants daff authorised human resources our laboratory manager has a masters degree and over 20 years experience in tissue culture. Sudhakaran and sivasankari have noticed that the nodal explants of oscimum basilicum showed maximum shootlet formation compared to apical bud53.
In vitro propagation and comparative phytochemical. Pdf genetic characterization and in vitro propagation of. Indian journal of biotechnology vol 5, january 2006, pp 112116 in vitro flowering and rapid propagation of vitex negundo l. In vitro propagation and conservation of useful endangered. Micropropagation of rare and threatened medicinal plant. Studies on propagation biology of four and phenology of one medicinal plants balcha abera a dissertation submitted to the school of graduate studies of addis ababa university in partial fulfillment of the requirement for the degree of doctor of philosophy in plant physiology january, 2009. In vitro micropropagation of medicinal plants by tissue. Rapid propagation of the medicinal plant pinellia ternata by.
Justicia adhatoda, usually known as malabar nut, adulsa, adhatoda, vasa, vasaka. Commiphora wightii, endangered, medicinal plant, in vitro propagation, tissue culture, india introduction commiphora wightii ar. Biotechnology laboratory, institute of biological sciences university of rajshahi, rajshahi6205, bangladesh abstract context. Concepts of propagating medicinal plants using modern tools virendra singh institute of himalayan bioresource technology p o bag no 6, palampur hp 176 061 india plant propagation is the first phase of complete package of production technology of herbal. Pdf in vitro propagation of medicinal and aromatic. Studies on tissue culture of chinese medicinal plant. A protocol for micro propagation of three important high altitude medicinal plant species ficus cordata ssp. Various concentrations of 6benzyl in vitro plantlet production has been achieved on 0. In vitro clonal propagation is an efficient means of exsitu conservation of plant diversity and it assists in sustainable leguminous liana, maintenance of the present day rapidly dwindling germplasm on long term basis, especially for the medicinal plants. To maintain humidity, the plants were watered periodically twice a day. In vitro propagation of plants holds great promise for conservation and enhancement of valuable medicinal plants.
Biotechnological approaches, specifically plant tissue culture plays a vital role in search for alternatives to production of desirable medicinal compounds from plants. In vitro propagation of a high value medicinal plant. High frequency bud break and fast development of shoots were induced on murashige and skoogs basal medium supplemented with 27. Medicinal plant, justicia, kfaplus, in vitro, clonal propagation introduction since ancient times, mankind has been dependent on plants for food, feed, flavours, medicine and many other uses. Less side effects, better compatibility and only accessible cure for some diseases makes the herbal drugs an ideal remedy for treatment of the diseases karim et al. Pdf tissue culturing of medicinal plants is widely used to produce active compounds for herbal and pharmaceutical industries. In vitro propagation using axillary buds has been reported in different species.
The bulbils in the in vitro plants were smaller than those from nature propagation, as the in vitro plants were still small at that time. Also, many medicinal plant species are disappearing at an alarming rate due to rapid agricultural and urban development, uncontrolled deforestation and indiscriminate collection. So tissue culture is a best way to conserve the medicinal plant and also the mass production of the medicinal plants. Hplc analysis of tissue culture raised plants grown in the greenhouse have shown significantly higher levels of the active compounds compared to wild types and commercial crude drug, demonstrating the. It is a traditional ayurvedic medicinal plant belonging to the family fabaceae. Micropropagation of plants of medicinal and pesticide importance micropropagation techniques have improved propagation of medicinal and pesticidal plants table 2. First, the establishment of in vitro propagation system for caper capparis spinosa l and lavender lavandula dentata l. Brazil has a great number of medicinal plants species distributed along.
In vitro micropropagation of medicinal plants by tissue culture. In vitro propagation and approaches for metabolites. Micropropagation can be used for largescale multiplication of essential oil producing plants thus avoiding an overexploitation of natural. In vitro propagation is an alternative method of propagation which can aid its conservation. Numerous factors are reported to influence the success of in vitro propagation of different medicinal plants and, therefore, it is unwise to define any particular reason for the general micropropagation of medicinal plants. Pdf in vitro micropropagation of medicinal plants by tissue. Concepts of propagating medicinal plants using modern tools. Micropropagation of maps is a powerful tool to conserve rare, threatened, and valuable maps, and to massively produce highvalue plant material for. This is the first report on ex vitro root induction in this plant with very good rate of response. In addition, inappropriate use wrong plant parts, dose, frequency, route of administration, preparation, etc. Combinations of in vitro propagation techniques fay, 1992 and. In vitro propagation of an endangered medicinal herb.
In vitro propagation and acclimatization of lippia. In the present days many medicinal plants are produce inin vitroconditions. Explants establishment and shoot initiation a simple and effective protocol was developed for the micropropagation of in vitro plectranthus amboinicus. The advantage of micropropagation system over conventional seed or vegetative propagation is that it is possible to quickly multiply plants with desirable genotype bonga 1987. Cleome rutidosperma has been used in indian ayurvedic medicine for the treatment of a wide number of. Pdf in vitro micropropagation of medicinal plants by. Micropropagation can be used for largescale multiplication of essential oil producing plants thus avoiding an overexploitation of natural resources. In vitro micropropagation of a valuable medicinal plant.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. In vitro propagation of the medicinal plant ziziphora tenuior l. Invitro clonal propagation of an indian medicinal plant. In vitro culture of aromatic and medicinal plants is a useful technique to produce rapidly and in small spaces a large amount of plant material, avoiding the damage and the extinction of natural field grown plants 1. In tribal name of justicia adhatoda known as kala basa encyclopedia, 20. In vitro conservation and propagation of medicinal plants. Chaturvedi et alcloning of medicinal plants 939 table 1. Rapid propagation of the medicinal plant pinellia ternata. The nature of the explant, seedling age, medium type, plant growth regulators, complex extracts casein hydrolysate, coconut milk, malt extract and yeast extract and antioxidants activated charcoal, ascorbic acid, citric acid and polyvinylpyrrolidone markedly influenced in vitro propagation of gymnema sylvestre. Poor seed setting and germination and slow growth in conventional vegetative propagation are major constraints in the largescale cultivation of this commercially important medicinal plant.
Applications of phytochemical and in vitro techniques for. Studies on propagation biology of four and phenology of one medicinal plants balcha abera a dissertation submitted to the school of graduate studies of addis ababa university in partial fulfillment of the requirement for the degree of doctor of philosophy in plant. To my delight and i am sure of anyone who picks up. In order to micro propagation and resolve its endangered experiment was done in biotechnology laboratory of sari agricultural and natural resources university in. In vitro propagation of endangered medicinal plantcommiphora. In vitro propagation of gentiana scabra bunge an important. In vitro propagation of some important chinese medicinal. In vitro propagation of the medicinal plant plumbago.
Protocols for in vitro cultures and secondary metabolite. Considerable progress has been made in the in vitro propagation of medicinal plants with few seeds andor low germination rate sarasan et al. In vitro clonal propagation of vulnerable medicinal plant. In vitro regeneration protocol for artificial seed production in an important medicinal plant mentha arvensis l m s islam and m a bari. Characterization is the first step in the conservation and utilization of indigenous genetic resources. We also highlight the need for the utilization of this technology for. In vitro culture offers many advantages for the creation of germplasm banks of living, growing medicinal plant tissues, mass propagation, and longterm storage in a suspended physiological state.